Guillaume received his DVM degree from the National Veterinary College of Alfort (Paris, France) in 2001, and pursued a residency program in Veterinary Ophthalmology from 2003 to 2006. He is a Diplomate of the ECVO since 2012. He worked until 2012 as a clinical associate professor at the Ophthalmology Unit of the National Veterinary College of Alfort, France. He currently works at the Fregis Veterinary Hospital in Arcueil-Paris, a multi-disciplinary private referral practice.